Ø UHS SYLLABUS TOPICS:
1- Cell Biochemistry
- Introduction to biochemistry: An overview of biochemistry and its significance in medicine.
- Biochemical composition and functions of cell: Organization and composition of eukaryotic and prokaryotic cells (only biochemical aspects)
- Cell membranes (biochemical composition)
- Membrane phenomena: Transport of substances across the cell membrane via active (primary and secondary active) transport; diffusion (simple and facilitated), and vesicle-mediated transport (phagocytosis, endocytosis, and exocytosis); Gibbs-Donnan equilibrium, osmosis and osmotic pressure
- Membrane receptors and other biologically important regulatory and catalytic membrane-bound proteins like G-proteins, adenlylyl cyclase, phospholipase.
- Basic methods to study cell biochemistry: Centrifugation, ultracentrifugation, radioimmunoassay, ELISA (enzyme-linked imnunosorbent assay); chromatography; electrophoresis, spectrophotometry, and pH metry.
2- Water, pH and buffers
- Ionization of water; weak acids and bases
- pH and pH scale: Concept of pH and related topics (determination of pH), and concept of pl (isoelectric pH)
- pKa value, dissociation constant (Ka), and titration curve of weak acids
- Determination of pH of buffer: Henderson-Hasselbalch equation and its applications (derivation not required).
- Body buffer systems (bicarbonate, ammonia, phosphate, and proteins) and their mechanism of action.
Ø TABLE OF SPECIFICATION:
· SEQs : 2.5 marks
· MCQS : 03 marks
Ø BOOKS:
· Lippincott
· HARPER illustrated biochemistry
· Medical biochemistry by MUSHTAQ
Ø SYLLABUS PREPARATION GUIDELINE:
- For Cell, read CHAPTER 1 from MUSHTAQ. Just give a read to cell organelles. These are not much important.
- For Cell Membrane, do it from CHAPTER 40 of HARPER.
- For membrane phenomenon, read from MUSHTAQ CHAPTER 2. This is an important topic.
- G Proteins can be done from HARPER CHAPTER 42 (don’t do the whole CHAPTER. Just Table 42.3, 42.4 . Also figure 42.6 and 42.8 with their respective topics) and lippincott ( Page no. 95, 205 figure 17.8 and Page no. 311). (VVVVVVVV.IMP)
- For Separation Techniques, you can do from MUSHTAQ CHAPTER 2 Page no. 20,27 and from CHAPTER 3 page 104.
- For pH and water, do from CHAPTER 2 from MUSHTAQ.
- For acid base balance and buffer system, do from MUSHTAQ volume 2 CHAPTER 9 OR you can do it from SATYANARAYANA CHAPTER 21 page 468 but there are some mistakes in this book.
Ø IMPORTANT :
· MCQs :
1. Numerical problems related to pH and pOH.
2. Examples of membrane transport phenomenon.
3. Buffer systems of body and determination of pH of buffers.
· SEQs :
1. Function of SER (CLINICAL)
2. Lipid rafts and caveole
3. Types of G proteins
4. Structure and composition of plasma membrane
5. Difference between lysosomes and peroxisomes
6. Disorders of alkalosis and acidosis (CLINICAL)
7. Three lines of defence against pH
8. Renal mechanisms along with diagrams.
· VIVA VOICE :
1. Methods of cell separation techniques
2. Types of G PROTEINS
3. Blood buffer systems
4. Types of transport
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